This page gives overall advice on how to assemble BlueSCSI V2 DB25 Kits. These kits already have the surface-mount soldering completed. All that remains to be done are the through-hole connectors.
First important note: All headers must be installed with the short end through PCB, and long end sticking up away from the board. The case won’t fit, and the jumpers won’t work, if installed incorrectly.
Second important note: Pay attention to the “USB” marker on the PCB. It points to where the USB port of the Raspberry Pi Pico module should be located. See pictures below for reference.
Third Important Note: Pay attention to silkscreen markings for the DB25 connector.